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The objective of the white paper is to present the difference in efficacy between an action on single wavelengths of the visible spectrum and their action on bacteria, particularly at 405nm, compared to a recent technique, Biovitae’s, that uses LED irradiation with a combination of multiple interfering waves of the visible spectrum with a main peak in the 400- 420nm range. This multifrequency wavelength has shown wide efficacy on both bacteria and viruses, including, for the first time, its ability to inactivate SARS-CoV-2 . In this work, we examine what reactions may occur in microorganisms when they are irradiated with a flux of photons consisting of multiple combined wavelengths. We do so by investigating some selected mechanisms that, through the irradiation of visible light, can contribute to the eradication process of bacteria and viruses.